Proteomic Multiplexer

My work at Proteowise was on a proteomics multiplexer with the ability to test complex cell lysates for the presence of thousands of proteins 100x faster than conventional methods. I refined the design for a highly complex microfluidic cartridge, and I managed the interdisciplinary team responsible for designing, manufacturing, and quality testing our beta instruments.

Images of the perfusion device and its components are protected by existing non-disclosure agreements.

Tools used

  • SolidWorks
  • 3D printer
  • Laser cutter
  • Microscopes
  • Wet lab
  • Electronics fabrication

Technologies implemented

  • Gel electrophoresis
  • ELISA
  • Sensing of pressure, flow rate, fluid levels, voltage, current, and temperature
  • Simultaneous control of 9 stepper motors

Product function

  • Perform gel electrophoresis on complex cell lysate samples
  • Transfer proteins onto proprietary membrane
  • Move polyacrylamide gel and proprietary membrane through microfluidic cartridge without damaging either or compromising fluidic integrity
  • Circulate 9 reagents in addition to 50 antibodies targeting specific proteins
The Proteowise “Hyperblot”